3D Skeletal muscle organoids as platforms for the study of muscle diseases and drug screening

Skeletal muscle is a highly organized, striated tissue representing approximately 40% of the adult human body. The main function of this tissue is to produce contractile force (CF), which is necessary for locomotion, respiration, and metabolic processes. Recent advances in tissue engineering have driven the development of in vitro skeletal muscle platforms that recapitulate key structural and functional properties of native tissue. These systems serve as powerful tools for investigating muscle development, regeneration, and the pathological mechanisms underlying disorders such as Duchenne muscular dystrophy and age-related fibrosis. Our research is focused on the development of physiologically relevant in vitro skeletal muscle models using a combination of bioengineering techniques, as well as the implementation of different tools to evaluate their structural organization and functional performance.